DORIVOR VA XO‘JALIK AHAMIYATIGA EGA O‘SIMLIKLAR (QALAMPIR, ALOE, GERAN) ENDOFIT ZAMBURUG‘LARINING IKKILAMCHI METABOLITLARI AJRATIB OLISH, ANTIBAKTERIAL TESTLAR TAHLILI
DOI:
https://doi.org/10.54613/ku.v17i.1368Keywords:
endofit zamburug‘lar, ikkilamchi metabolitlar, antibakterial faollik, Capsicum annuum, aloe vera, Pelargonium spp.Abstract
Ushbu maqola dorivor va xo‘jalik ahamiyatiga ega bo‘lgan qalampir (Capsicum annuum), aloe (Aloe vera) va geran (Pelargonium spp.) o‘simliklarining turli organlarida (ildiz, poya va barg) uchraydigan endofit zamburug‘larni ajratib olish, ularning ikkalamchi metabolitlarini ekstraksiya qilish hamda turli gram-musbat va gram-manfiy bakteriyalarga nisbatan antibakterial faolligini baholashga bag‘ishlangan.
Tadqiqotda endofit zamburug‘lar klassik mikrobiologik usullar yordamida izolyatsiya qilindi, Chapek-Dox muhitida yetishtirilib, etil asetat orqali ikkalamchi metabolitlari ajratib olindi. Olingan ekstraktlarning antibakterial ta’siri Kirby–Bauer disk-diffuziya usuli yordamida Escherichia coli, Staphylococcus aureus, Bacillus subtilis va Pseudomonas aeruginosa shtammlariga nisbatan baholandi. Natijalar barg va poya to‘qimalaridan ajratilgan endofitlar ildiz izolyatlariga nisbatan yuqori metabolit hosildorligiga ega ekanini ko‘rsatdi. Ayniqsa, qalampir, aloe va geran barglaridan olingan ayrim izolyatlarda yuqori darajada bioaktiv ikkalamchi metabolitlar to‘planishi qayd etildi.
Olingan natijalar endofit zamburug‘larning yangi tabiiy antibakterial birikmalar manbai sifatida katta ilmiy va amaliy ahamiyatga ega ekanini tasdiqlaydi.
Foydalanilgan adabiyotlar:
1. Jones, J. T., Haegeman, A., Danchin, E. G. J., Gaur, H. S., Helder, J., Jones, M. G. K., Kikuchi, T., Manzanilla-López, R., Palomares-Rius, J. E., Wesemael, W. M. L., & Perry, R. N. (2013).
2. Nicol, J. M., Turner, S. J., Coyne, D. L., den Nijs, L., Hockland, S., & Maafi, Z. T. (2011). Current nematode threats to world agriculture. In Genomics and Molecular Genetics of Plant-Nematode Interactions (pp. 21–43). Springer.
3. Hallmann, J., Quadt-Hallmann, A., Mahaffee, W. F., & Kloepper, J. W. (1997). Bacterial endophytes in agricultural crops. Canadian Journal of Microbiology, 43(10), 895–914.
4. Petrini, O. (1991). Fungal endophytes of tree leaves. In J. H. Andrews & S. S. Hirano (Eds.), Microbial Ecology of Leaves (pp. 179–197). Springer.
5. Poveda, J. (2020). Biological control of plant-parasitic nematodes by fungal endophytes: mechanisms of action and prospects for use. Frontiers in Microbiology, 11, 992.
6. Yan, X.-N., Sikora, R. A., & Zheng, J.-W. (2011). Potential use of cucumber (Cucumis sativus L.) endophytic fungi as seed treatment agents against root-knot nematode Meloidogyne incognita. Journal of Zhejiang University Science B, 12(3), 219–225.
7. Sikandar, A., Zhang, M., Wang, Y., Zhu, X., Liu, X., Fan, H., & Xie, X. (2020). In vitro evaluation of Penicillium chrysogenum Snef1216 against Meloidogyne incognita in cucumber. Scientific Reports, 10, 8342.
8. Yan, X.-N., Chairin, T., Pornsuriya, C., Soytong, K., & Hyde, K. D. (2021). Evaluation of a newly identified endophytic fungus, Trichoderma phayaoense, for plant growth promotion and biological control. Frontiers in Microbiology, 12, 661948.
9. Hallmann, J., Quadt-Hallmann, A., Mahaffee, W. F., & Kloepper, J. W. (1997). Bacterial endophytes in agricultural crops. Canadian Journal of Microbiology, 43(10), 895–914.
Downloads
Published
Iqtiboslik olish
Issue
Section
License
Copyright (c) 2026 QO‘QON UNIVERSITETI XABARNOMASI
This work is licensed under a Creative Commons Attribution 4.0 International License.